WebMitotracker Red CMXRos was dissolved in M9 to a stock concentration of 1 µg/µl and added to late L4 stage col-121 worms in liquid culture at a final concentration of 1 µg/ml for 24 hr. After treatment, worms were washed in M9 five times, and placed on regular NGM plates containing only OP50 E. coli for 1 hr to clear any residual dye present on their … Web8 mrt. 2024 · Mitophagy detection assay The mitophagy detection kit was used to detect mitophagy in NPCs. The NPCs were seeded in the logarithmic growth period on a 6-well plate covered with cell slides at 2 × 10 5 per well. The NPCs were cultured at 37 °C overnight under 5% CO 2 saturated humidity and treated as depicted above.

LysoTracker and LysoSensor Probes - Thermo Fisher Scientific

WebThermo Fisher Scientific - US Web19 okt. 2024 · To confirm the significance of the bioinformatics observations, we tested YME1L expression in local human glioma tissues (“T”) and surrounding normal brain (“N”) tissues, from a total of 16 grade III–IV glioma (HGG) patients (see our previous studies such as Liu et al. (2024) and Wang et al. (2024).The real-time quantitative reverse … frontline education create id

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Web1 aug. 2011 · MitoTracker is a commercially available fluorescent dye (Invitrogen/Molecular Probes) that, like the aforementioned dyes, labels mitochondria within live cells utilizing … http://www.warbio.cn/news/show-1372.html WebDescription: High throughput cell-based assay for measuring mitochondrial mass. Assay: MitoTracker® Dye (Green 488, Red 549 or Far Red 650) Format: 96-well plates … frontline education elizabeth public schools